Premium
ATPase‐Associated Oligomycin Resistance in Acanthamoeba castellanii 1
Author(s) -
SEILHAMER JEFFREY J.,
BYERS THOMAS J.
Publication year - 1982
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1982.tb05419.x
Subject(s) - oligomycin , atpase , biology , chemistry , microbiology and biotechnology , biochemistry , enzyme
. The multiplication rate of “wild‐type” (WT) populations of Acanthamoeba castellanii was inhibited 50% by ∼3 μg otigomycin/ml; Oli R2 an oligomycin resistant cell line, required ∼27 μg/ml for the same inhibition. ATPase solubilized from Oli R2 mitochondrial fractions required 3–10‐fold higher concentrations of oligomycin than did identical WT fractions to achieve 50% inhibition of activity. Resistance was correlated with altered mitochondrial ATPase sensitivity to oligomycin.