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Radioiodination of Parasite Antigens with 1,3,4,6,‐Tetrachloro‐3α,6α‐diphenylglycoluril (IODOGEN): Studies with Zygotes of Plasmodium gallinaceum
Author(s) -
HOWARD R. J.,
KAUSHAL D. C.,
CARTER R.
Publication year - 1982
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1982.tb02891.x
Subject(s) - plasmodium gallinaceum , lactoperoxidase , zygote , coomassie brilliant blue , chemistry , antigen , reagent , polyacrylamide gel electrophoresis , chromatography , biochemistry , biology , staining , enzyme , plasmodium falciparum , malaria , peroxidase , genetics , immunology , gene , gametocyte , embryogenesis
The iodinating reagent 1,3,4,6,‐tetrachloro‐3α,6α‐diphenylglycoluril (IODOGEN 3 ) was used to label antigens on zygotes of Plasmodium gallinaceum with parallel studies using lactoperoxidase‐catalyzed radioiodination for comparison. Proteins labeled by the IODOGEN method are most probably on the surface of the zygote, as the pattern of labeled proteins analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis was very similar to the pattern of lactoperoxidase‐labeled proteins. Furthermore, the labeled proteins represented only a subset of the total Coomassie Blue‐stained proteins. The radioiodinated zygote proteins were immuno‐reactive after IODOGEN or lactoperoxidase labeling. The IODOGEN method is technically much more simple than the lactoperoxidase method and does not require the addition of extraneous proteins or H 2 O 2 . The advantages of IODOGEN labeling, together with the essential equivalence of results obtained by these two, methods, make the IODOGEN method attractive for labeling parasite antigens in general.