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Growth of Isolated Amastigotes of Trypanosoma cruzi in Cell‐Free Medium 1
Author(s) -
VILLALTA FERNANDO,
KIERSZENBAUM FELIPE
Publication year - 1982
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1982.tb01338.x
Subject(s) - trypanosoma cruzi , amastigote , chemistry , radiochemistry , virology , biology , leishmania , computer science , parasite hosting , world wide web
Amastigotes of Trypanosoma cruzi were purified from overlays of infected Vero cell cultures by centrifugation over a discontinuous gradient of metrizamide. Pure amastigote preparations were usually recovered from the pellet under the layer of specific gravity 1.086. The isolated amastigotes grew in cell‐free ML‐15HA medium. Growth rate for the different strains of T. cruzi were in the order Y > Tulahuan > CL. The generation time of amastigotes in ML‐15HA medium was 16.8, 18.0, and 26.4 h for the Y, Tulahuen, and CL strains, respectively, in the presence of 5% CO 2 ) and 16.8, 31.2, and 36.4 h, respectively, in the absence of CO 2 . Intracellular amastigotes did not differ ultrastructurally from amastigotes from either the density‐gradient fractionation or culture in cell‐free medium.