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Formation of 2‐Aminobutanoic Acid from Threonine and Methionine by Mixed Rumen Ciliate Protozoa
Author(s) -
ONODERA RYOJI,
USHIJIMA TAKAYUKI
Publication year - 1982
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1982.tb01332.x
Subject(s) - methionine , rumen , protozoa , threonine , ciliate , incubation , biology , biochemistry , bacteria , amino acid , chemistry , microbiology and biotechnology , phosphorylation , serine , ecology , genetics , fermentation
Precursors of 2‐aminobutanoic acid (2‐ABA), found in the incubation medium of mixed rumen ciliate protozoa, were examined with washed or starved bacteria‐free ciliates. Threonine and methionine strongly stimulated the formation of 2‐ABA. Formation of 2‐ABA by direct conversion of threonine and dethiomethylation of methionine was confirmed by radiotracer experiments with [U‐ 14 C]L‐threonine and [carboxyl‐ 14 C] and [methyl‐ 14 C]L‐methionine.