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Local and Temporal Pattern of Con A‐Binding Site Aggregation During Conjugation in Euplotes vannus (Ciliophora, Hypotrichida) 1
Author(s) -
LUEKKEN WOLFGANG W.,
BREER HEINZ,
HARTKKEMEYER MARTINA
Publication year - 1981
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1981.tb05312.x
Subject(s) - paraformaldehyde , glycoconjugate , chemistry , biophysics , ciliata , fluorescence , microbiology and biotechnology , biology , biochemistry , genetics , optics , protozoa , physics , organic chemistry
. Conjugating cells of Euplotes vannus (syngens Naples and Barcarès) were investigated for Con A‐binding sites by means of fluorescence microscopy. Cells fixed with 0.4% paraformaldehyde and incubated with 15 μg/ml FITC‐Con A showed a distinct region of strong fluorescence, the size, shape and localization of which characteristically changed during the course of conjugation, first appearing at courtship stage (cells contact without forming pairs) and vanishing soon after the migration pronuclei have been exchanged, but before cells have separated. Con A binding and conjugation were blocked by cyclohexamide and α‐galactosidase. Con A‐binding was also inhibited by α‐mannosidase, α‐glucosidase and β‐galactosidase without affecting conjugation except a delay of pair formation. The results suggest an involvement of newly formed or translocated glycoconjugates in cell pairing during conjugation.