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Phagocytosis of Trypanosoma cruzi by Human Polymorphonuclear Leukocytes 1
Author(s) -
RIMOLDI MARÍA TERESA,
OLABUENAGA SUSANA E.,
BRACCO MARÍA M. DE E.
Publication year - 1981
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1981.tb02864.x
Subject(s) - phagocytosis , trypanosoma cruzi , lysis , effector , uridine , antibody , opsonin , chemistry , zymosan , microbiology and biotechnology , biology , biochemistry , immunology , in vitro , rna , parasite hosting , world wide web , computer science , gene
Phagocytosis of culture forms of Trypanosoma cruzi was assayed by a radioisotopic method. Purified polymorphonuclear leukocytes (PMN) were mixed with 3 H‐uridine‐labeled T. cruzi epimastigotes in the presence or absence of anti‐ T. cruzi antibodies. The reaction was stopped by adding N‐ethyl‐maleimide, and noningested parasites were lysed by complement. The percentage of radioactivity incorporated into the PMN pellet was recorded. The phagocytosis reaction was rapid, yielding maximum incorporation at 30 min at which point the radioactivity associated with the PMN cells decreased through release of the isotope to the supernatant. The degree of incorporation of radio‐labeled parasites was a function of the effector/target cell ratio and the antibody concentration. The method is suitable for the quantitative determination of phagocytosis of T. cruzi by normal PMN.