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Concanavalin A‐Sepharose Chromatography of Hexosaminidase Isozymes Secreted By Tetrahymena *
Author(s) -
VICK G. WESLEY,
BLUM J. J.
Publication year - 1980
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1980.tb04691.x
Subject(s) - isozyme , concanavalin a , biochemistry , sepharose , biology , affinity chromatography , hexosaminidase , tetrahymena , glycoprotein , microbiology and biotechnology , enzyme , chromatography , chemistry , in vitro
Tetrahymena pyriformis strain HSM secretes 4 isozymes of hexosaminidase. Purified isozymes B 1 and B 2 are eluted from the void volume of a concanavalin A‐Sepharose column, suggesting that they are not glycosylated. Purified isozymes A 1 and A 2 bind to the column and are eluted at ∼0.1 M α‐methylmannoside, suggesting that these isozymes are glycoproteins. In agreement with earlier deductions based on a differential kinetic assay for the A and B isozymes, the elution pattern of hexosaminidase activity from material secreted by cells grown to early and late stationary phase was consistent with these secretions containing primarily the B and the A isozymes, respectively.