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Trypanosoma Cruzi: Pattern of Rna Synthesis Following Infection of Vertebrate Cells
Author(s) -
CRANE MARK,
DVORAK JAMES A.
Publication year - 1980
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1980.tb04273.x
Subject(s) - trypanosoma cruzi , amastigote , rna , intracellular , biology , nucleic acid , extracellular , cell , microbiology and biotechnology , biochemistry , parasite hosting , leishmania , gene , world wide web , computer science
SYNOPSIS. the pattern of RNA synthesis of intracellular Trypanosoma cruzi amastigotes, immediately following infection of Lesch‐Nyhan human fibroblasts, was studied by autoradiography. Amastigote RNA synthesis, determined by [ 3 H]guanine incorporation, was not detected until 2 h after infection. At 8 h postinfection more than 90% of intracellular amastigotes were labeled. It was verified that extracellular trypomastigotes also synthesized RNA. Therefore it was concluded that, if RNA is required for trypomastigote‐to‐amastigote transformation, this nucleic acid is already present in the trypomastigotes before infection of the vertebrate cell. It is probable that the RNA synthesized by amastigotes during the prereplicative lag period (the period between initial infection and the onset of DNA synthesis) is required for intracellular growth and reproduction.