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Morphologic and Biochemical Characterization of Crithidia Brasiliensis Sp. N. *
Author(s) -
SÁ MARIA F. G.,
SÁ CEZAR M.,
VERONESE MARCO A.,
FILHO SPARTACO ASTOLFI,
GANDER EUGEN S.
Publication year - 1980
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1980.tb04248.x
Subject(s) - crithidia , biology , evolutionary biology , zoology , botany , protozoa
SYNOPSIS. A trypanosomatid with a choanomastigote stage and, therefore, belonging to the genus Crithidia , was isolated in culture from the alimentary tract of the hemipteran genus Zelus. the trypanosomatid was able to grow at 37 C, a characteristic reported to date from only 2 other members of Crithidia, C. hutneri and C. luciliae thermophila. Subsequently, the flagellate was cloned for biochemical studies which involved cleaving of kDNA by restriction endonucleases and analyses of the isoenzyme and histone patterns. In all the attributes revealed by the foregoing methods, the organism from Zelus differed from the latter 2 congeneric species. On these and morphologic grounds, this organism appears to belong in a new species for which the name Crithidia brasiliensis sp. n. is proposed.