Premium
Toxicity of Cadmium to Amoeba Proteus : A Biochemical Approach
Author(s) -
AlAtia G. R.
Publication year - 1980
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1980.tb04242.x
Subject(s) - amoeba proteus , cytosol , cadmium , chemistry , metalloprotein , amoeba (genus) , biochemistry , mytilus , metallothionein , cysteine , biology , microbiology and biotechnology , enzyme , cytoplasm , ecology , organic chemistry
SYNOPSIS The cadmium ion (Cd 2+ ) was accumulated by Amoeba proteus in all cellular fractions, the highest level being associated with the cytosol fraction. On gel separation of the cytosol fraction, Cd‐binding protein appeared in 2 peaks: one >45,000 MW (peak I) and the other 12,000 MW (peak II). Added cysteine increased the total Cd 2+ taken up by the cells and resulted in disproportionate increase of Cd incorporated into the Cd‐binding protein of peak II. the Cd‐binding protein of peak II is analogous to the low‐MW, Cdbinding proteins in Anacystis nidulans, Mytilus edulis , and to the metalloprotein of some vertebrates.