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Replication of Nosema Algerae In Three Insect Cell Lines
Author(s) -
STREETT D. A.,
RALPH DAVID,
HINK W. F.
Publication year - 1980
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1980.tb04239.x
Subject(s) - trichoplusia , biology , spore , nosema , cell culture , virology , in vitro , microbiology and biotechnology , botany , lepidoptera genitalia , noctuidae , genetics , microsporidia
SYNOPSIS Nosema algerae , a microsporidan parasite of anopheline mosquitoes, was successfully replicated in 3 insect cell culture lines: Trichoplusia ni (TN‐368); Heliothis zea (IPLB‐1075); and Mamestra brassicae (IZD‐Mb‐0503). Infectious spores were produced in vitro. Spores were observed at 48 h postinfection, and some cells were filled with sproes by 72 h. The number of parasites per cell increased with time. At 72 h postinfection, the infection rates for the 3 cell lines ranged from 23 to 32%. Infected cell lines were subcultured, and by the 6th passage spore production had ceased.