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The Methylated Purines of Tetrahymena pyriformis Transfer Ribonucleic Acid *
Author(s) -
KEEGAN FRANK P.,
BERECH JOHN
Publication year - 1979
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1979.tb04660.x
Subject(s) - tetrahymena pyriformis , chromatography , chemistry , column chromatography , tetrahymena , guanine , purine , acid hydrolysis , purine metabolism , paper chromatography , hydrolysis , biochemistry , nucleotide , enzyme , gene
SYNOPSIS. By phenol extraction and DEAE cellulose column chromatography, tRNA was isolated from Tetrahymena pyriformis strain GL. Following acid hydrolysis of the tRNA, the methylated purine content was determined by Dowex 50 column chromatography and paper chromatography. The most abundant methylated guanine derivative was found to be N 2 ‐DMG. Also present were 1‐MG, N 2 ‐MG, and 7‐MG. The most abundant methylated adenine was found to be 1‐MA; no 2‐MA was detected. Small amounts of the N 6 ‐methyladenines were detected.