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Synchronization of Division in Vitamin B 12 ‐Starved Euglena gracilis
Author(s) -
SHEHATA T. E.,
KEMPNER E. S.
Publication year - 1979
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1979.tb04209.x
Subject(s) - euglena gracilis , cell division , biology , vitamin b12 , division (mathematics) , dna synthesis , euglena , cell cycle , dna , cell , microbiology and biotechnology , biochemistry , gene , arithmetic , mathematics , chloroplast
SYNOPSIS Vitamin B 12 deficiency arrests cell division in Euglena gracilis. B 12 starvation for short periods made it possible to induce synchronous growth by addition of the vitamin. Culture conditions were established to optimize replenishment synchrony. The DNA content of E. gracilis in steady state culture and vitamin B 12 deficiency culture was measured by flow cytofluorometry and was consistent with colorimetric determinations. The cell volume and DNA distributions of E. gracilis in synchronous culture were analyzed and the sequential changes during the division cycle were computed. Synchronous culture permits more definitive studies of shifts in cell volume and DNA distributions, in which the biochemical events required for cell division are presumably synchronized.