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Purification of Plasmodium lophurae Exoerythrocytic Merozoites by an Ion Exchange Column *
Author(s) -
HOLLINGDALE MICHAEL R.,
KILEJIAN ARAXIE
Publication year - 1979
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1979.tb04207.x
Subject(s) - chromatography , elution , ion exchange , parasite hosting , plasmodium (life cycle) , sodium , biology , chemistry , electron microscope , ion , physics , organic chemistry , world wide web , computer science , optics
SYNOPSIS Exoerythrocytic merozoites of Plasmodium lophurae grown in embryonic turkey brain cells were successfully separated from host cell material by elution from a DEAE‐cellulose column at ionic strength 0.22. Purity of parasite samples was assessed by sodium dodecyl sulphate acrylamide gel electrophoresis and electron microscopy. Increasing the ionic strength gave greater recoveries of merozoites, but host cell contamination increased.