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Extrusion of DNA from the Macronucleus of Tetrahymena pyriformis GL
Author(s) -
LYKKESFELDT ANNE E.
Publication year - 1979
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1979.tb04200.x
Subject(s) - macronucleus , tetrahymena pyriformis , dna , tetrahymena , cell division , biology , dna synthesis , thymidine , bromodeoxyuridine , dna replication , microbiology and biotechnology , cell growth , cell , genetics
SYNOPSIS Administration of the thymidine analog 5‐bromodeoxyuridine to exponentially growing cultures of Tetrahymena pyriformis GL in chemically defined medium results in inhibition of cell multiplication by at least one generation before DNA synthesis stops. Cell multiplication can be restored in these cultures, if they are transferred to fresh growth medium, but although most of the cells in the culture contain close to a G2‐amount of DNA, a full DNA replication round is a prerequisite for renewed cell multiplication. Large extrusion bodies are found at the first division after transfer to fresh growth medium. Autoradiographic analysis has revealed that the DNA in the extrusion body is a representative of the DNA in the macronucleus indicating a random distribution of DNA between daughter nuclei and extrusion body.