Premium
Plasmodium falciparum in Culture: Improved Continuous Flow Method *
Author(s) -
TRAGER WILLIAM
Publication year - 1979
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1979.tb02747.x
Subject(s) - parasitemia , plasmodium falciparum , continuous flow , flow (mathematics) , biology , malaria , microbiology and biotechnology , immunology , mechanics , physics
SYNOPSIS. A new design of flow vessel provides a method for continuous culture of P. falciparum in a settled layer of human erythrocytes with a slow flow of culture medium over them. The parasitemia is kept fluctuating from ˜ 1%, just after addition of fresh erythrocytes. to ˜ 10%, 2 or 3 days later. Each vessel provides each week 3 harvests, each containing ˜ 0.6–1 × 10 9 parasites.