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Mutants of Acanthamoeba castellanii Resistant to Erythromycin, Chloramphenicol, and Oligomycin
Author(s) -
SEILHAMER JEFFREY J.,
BYERS THOMAS J.
Publication year - 1978
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1978.tb04172.x
Subject(s) - chloramphenicol , oligomycin , mutant , microbiology and biotechnology , biology , ethidium bromide , cycloheximide , antibiotics , biochemistry , protein biosynthesis , dna , atpase , gene , enzyme
SYNOPSIS. Cell lines of Acanthamoeba castellanii resistant to erythromycin (Ery R ), chloramphenicol (Cap R ), and oligomycin (Oli R ) have been isolated. These may be the first such mutants for A. castellanii. These mutants have been phenotypically stable for 2 years, surviving storage and vegetative multiplication in the absence of drugs. Resistance was specific for each drug, but double mutants (e.g. Ery R Cap R ) were obtained by stepwise selection. Mutant frequencies were determined in multiwell plates; <10 colony forming units (CFU/10 5 amebas were observed in wild‐type populations 12 days after incubation in 500 μg Ery/ml, 2.5 mg Cap/ml, or 15 μg Oli/ml. After 30 days, averages of 100 CFU/10 5 amebas were observed in Ery and Cap, whereas, frequencies for Oli remained unchanged. Frequencies for Ery R and Cap R were consistent with rates of recovery from these drugs in batch cultures. We were unable to obtain spontaneous mutants resistant to cycloheximide, emetine, 5‐fluorodeoxyuridine, or ethidium bromide. Ery R , Cap R and Oli R could be mitochondrial mutants.