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Continuous Cultivation of Trypanosoma theileri at 37 C in Bovine Cell Culture *
Author(s) -
McHOLLANDRAYMOND LINDA E.,
KINGSTON NEWTON,
TRUEBLOOD MALCOLM S.
Publication year - 1978
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1978.tb03912.x
Subject(s) - subculture (biology) , fetal bovine serum , biology , trypanosoma , trypanosoma brucei , cell culture , microbiology and biotechnology , biochemistry , genetics , gene
SYNOPSIS.Trypanosoma theileri was cultivated at 37 C in bovine bone marrow cell culture through 50 consecutive subcultures. Medium 199, supplemented with Bacto‐peptone, vitamin B 12 , and fetal bovine serum, was utilized both for primary and continuous cultivation. The number of trypanosomes produced in culture averaged 8 × 10 6 (1–26 × 10 6 ) trypanosomes/ml. In each subculture the organisms divided as epimastigotes and transformed into trypomastigotes; a round form was observed during the stationary and declining phase of growth. Gradual changes such as increased generation time, size reduction, and decreased trypomastigote production were observed as subculturing progressed. Cultured trypanosomes were infective for the bovine through the 48th serial transfer and could be cultivated at 26 C.