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Effects of Methylglyoxal bis (Guanylhydrazone) on Trypanosomatid Flagellates: Inhibition of Growth and Nucleoside Incorporation in Trypanosoma brucei *
Author(s) -
CHANG K.P.,
STEIGER R. F.,
DAVE C.,
CHENG Y.C.
Publication year - 1978
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1978.tb03887.x
Subject(s) - trypanosoma brucei , spermidine , methylglyoxal , biology , trypanosoma , spermine , thymidine , nucleoside , biochemistry , in vitro , microbiology and biotechnology , virology , enzyme , gene
SYNOPSIS. Methylglyoxal bis (guanylhydrazone) (MGBG) at 0.5 m m had little effect in vitro on Blastocrithidia culicis, Crithidia oncopelti , and Leishmania spp., but completely inhibited growth of Trypanosoma brucei. Inhibition became irreversible after a 3‐h exposure of T. brucei culture procyclics. Treated organisms remained motile, but failed to divide. Polyamines, spermidine, and spermine, did not reverse the anti‐trypanosome action of MGBG (preloading of cells or concurrent administration). Two intraperitoneal injections of the drug at a concentration of 50 mg kg body weight at a 1‐day interval greatly reduced the parasitemia of T. brucei and T. congolense in rats. Trypanosome infections, however, relapsed and killed the animals in 6 days after treatment. It was evident from the results of tracer experiments with T. brucei that MGBG significantly lowered incorporation of [ 3 H]thymidine by culture procyclics and of [ 3 H]uridine by bloodstream forms; in both stages [ 3 H]leucine incorporation was only slightly inhibited. It is suggested that MGBG interferes with nucleoside incorporation by Trypanosoma and that its mode of action is different in bloodstream and culture procyclics.