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Ciliary Membranes and Mating Substances in Paramecium caudatum
Author(s) -
WATANABE TSUYOSHI
Publication year - 1977
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1977.tb04767.x
Subject(s) - paramecium caudatum , paramecium , mating , membrane , biology , chemistry , anatomy , microbiology and biotechnology , zoology , genetics
SYNOPSIS Cilia detached from mating reactive cells of Paramecium caudatum were fractionated for the purpose of identifying the structural component bearing mating substances. Purified axoneme fractions had no mating reactivity. The membrane fraction obtained by dialyzing against a solution of Tris‐EDTA (0.1 m m EDTA, 1 m m Tris‐HCI, pH 7.6) and 0.6 m KCI, and then by centrifuging over 40% (w/v) sucrose was strongly reactive. No mating reactivity was detected in the soluble fractions containing axonemal and matrix proteins. The results indicate that the mating substances in active from are localized only on the ciliary membranes.