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Subcellular Effects of Cytochalasin B and Dimethylsulfoxide on Paramecium aurelia *
Author(s) -
SIBLEY JANE T.,
PAUL MATTHEW D.,
HANSON EARL D.
Publication year - 1977
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1977.tb01022.x
Subject(s) - polysome , paramecium aurelia , chromatin , cytochalasin b , biology , dimethyl sulfoxide , biochemistry , chemistry , electron microscope , biophysics , microbiology and biotechnology , cell , ribosome , paramecium , rna , dna , physics , optics , gene , organic chemistry
SYNOPSIS.Paramecium aurelia syngen 4, stock 57 (sensitive) cultivated in Cerophyl infusion were exposed to cytochalasin B CB and dimethylsulfoxide (DMSO), the solvent for CB, to distinguish between the effects of these agents on a cellular system. DMSO significantly inhibited survival, fission rate, [ 3 H]leucine incorporation, and cell size. CB‐treated cells generally had slower division and poorer survival rates than cells exposed to the equivalent DMSO concentration, although the [3H]leucine incorporation was generally greater at the lower CB concentrations than for DMSO alone. As seen by electron microscopy and a new grycerination technic for observing polysomes, DMSO caused nuclear (nucleolar, chromatin) abnormalities as well as membrane degradation and polysomal breakdown; CB caused the formation of aberrant membrane structures and ribosomal tetramers, crystals, and tubes.