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N ‐methyl‐ N ′‐nitro‐ N ‐nitrosoguanidine and UV induced mutants of the dinoflagellate Crypthecodinium cohnii *
Author(s) -
TUTTLE† ROBERT C.,
LOEBLICH ALFRED R.
Publication year - 1977
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1977.tb00985.x
Subject(s) - dinoflagellate , mutant , biology , nitro , genetics , botany , chemistry , gene , organic chemistry , alkyl
SYNOPSIS. Mutant strains were chemically induced by treatment with N ‐methyl‐ N ′‐nitro‐ N ‐nitrosoguanidine (NTG) and UV irradiation. UV and NTG mutation rates were obtained that were both consistent with the organism being haploid. Three types of mutants were produced: (a) strains deficient in both β ‐ and γ ‐carotene, the only carotenoids found in the wild type; phenotypes include albinos (translucent, dull white, “snow white”) and cream‐colored on agar as compared to the yellow‐orange color of wild type colonies; (b) strains requiring adenine, guanine or cytosine in addition to the minimal medium for growth; (c) mutants that grow at a rate less than 40% of the wild type in minimal medium.