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Timing and Regulation of Nuclear and Cortical Events in the Cell Cycle of Tetrahymena pyriformis *
Author(s) -
SUHRJESSEN PETER B.,
STEWART JOHN M.,
RASMUSSEN LEIF
Publication year - 1977
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1977.tb00982.x
Subject(s) - tetrahymena pyriformis , tetrahymena , ciliate , cell cycle , biology , cell division , morphogenesis , cell , microbiology and biotechnology , chemostat , generation time , genetics , population , bacteria , gene , medicine , environmental health
SYNOPSIS. Using continuous flow cultures based on the chemostat principle, we varied the cell generation times of the ciliate Tetrahymena pyriformis strain GL, from 4.9 to 22.2 hr and studied various parameters of the cell cycle at 28 C. These included: the duration of the periods required for oral morphogenesis, macronuclear division, cell division, G 1 S, and G 2 . The size of individual cells was also measured. Independent of the growth rate, the period of oral morphogenesis occurred during the last 90 min of the cell cycle. In all cases macronuclear and cell divisions took place during the last part of these 90 min, and the final macronuclear separation occurred just before final cell separation. The S‐period increased slightly, while the G 1 and G 2 both increased in roughly the same relative proportion to the increasing generation times. Slowly growing cells (generation time 20.5 hr) were shorter but broader and somewhat larger in volume than quickly growing cells (generation time 4.9 hr).