Premium
Effect of Chloramphenicol on Bacterial Endosymbiotes in a Strain of Amoeba proteus *
Author(s) -
JEON K. W.,
HAH J. C.
Publication year - 1977
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1977.tb00979.x
Subject(s) - amoeba proteus , chloramphenicol , microbiology and biotechnology , strain (injury) , proteus , ultrastructure , amoeba (genus) , bacteria , biology , antibiotics , chemistry , anatomy , escherichia coli , genetics , gene
SYNOPSIS. The effect of chloramphenicol (CAP) on the bacterial endosymbiotes of a strain of Amoeba proteus was studied by growing the symbiotic amebae in media containing 0.5–1.6 mg/ml CAP for up to 4 weeks. Treatments with CAP caused such ultrastructural changes as expansion of the nuclear zone and deformation of symbiotes. The CAP treatment also damaged the mitochondria, e.g. disappearance of central and protrusion of peripheral cristae. Number of bacteria per ameba decreased to < 10% of control in CAP‐containing media, but no viable amebae became completely free of symbiotes. The resuts supported previous studies that amebae were dependent on endosymbiotes.