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Axenic Cultivation of Phytomonas davidi Lafont (Trypanosomatidae), a Symbiote of Laticiferous Plants (Euphorbiaceae) *
Author(s) -
MCGHEE R. BARCLAY,
POSTELL FRED J.
Publication year - 1976
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1976.tb03761.x
Subject(s) - euphorbiaceae , biology , axenic , botany , manihot esculenta , bacteria , genetics
SYNOPSIS.Phytomonas davidi (Trypanosomatidae) originally discovered by Lafont in 1909 on the island of Mauritius was rediscovered in Euphorbia cyathophora in Florida. Successful cultures were established in diphasic medium consisting of duck blood agar and modified Phillips’medium as overlay. Optimal growth was obtained when Mansour's medium was used as overlay and poorest growth when Cowperthwaite's medium buffered at pH 5.0 was utilized for this purpose. Marked changes tending toward choanomastigotes rather than the elongate twisted promastigotes were observed in cultures.