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The Immobilization Antigen of Paramecium aurelia is a Single Polypeptide Chain
Author(s) -
HANSMA HELEN G.
Publication year - 1975
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1975.tb05861.x
Subject(s) - paramecium aurelia , antigen , protease , paramecium , chemistry , biochemistry , microbiology and biotechnology , biology , enzyme , immunology
SYNOPSIS The immobilization antigen (i‐antigen) fraction of Paramecium aurelia syngen 4 is shown to contain a protease that is activated by mercaptoethanol. After the protease has been heat‐inactivated, the molecular weight of the i‐antigen (∼250,000 daltons) cannot be decreased by mercaptoethanol treatment. It is demonstrated that the i‐antigen is a single polypeptide chain. Reasons are also given why low molecular weight subunits were previously reported by other authors.