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Growth in vitro and Metabolism of Plasmodium vinckei chabaudi *
Author(s) -
COOMBS G. H.,
GUTTERIDGE W. E.
Publication year - 1975
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1975.tb05232.x
Subject(s) - plasmodium chabaudi , incubation , biology , parasite hosting , in vitro , biochemistry , adenosine , leucine , metabolism , microbiology and biotechnology , amino acid , immunology , plasmodium falciparum , malaria , parasitemia , world wide web , computer science
SYNOPSIS. An in vitro system, based on the rocker dilution technic, has been developed that supports intraerythrocytic growth of a rat‐adapted strain of Plasmodium vinckei chabaudi from ring to schizont stages; some reinvasion was obtained, although invariably, this was associated with a decrease in parasite numbers. Pertinent features were the very high buffer content of the medium and the low oxygen tension of the gaseous phase. Lactate production, glucose utilization, and 3 H‐leucine and 3 H‐adenosine incorporations were investigated for their suitability to monitor parasite growth. Throughout an 18‐hr incubation there was a continuous and increasing production of lactate and utilization of glucose, which correlated well with the development of the parasites from ring to schizont stages. During the same period, there was a low but continuous and increasing incorporation of 3 H‐leucine into parasite protein. However, 3 H‐adenosine was incorporated only for the 1st hr of incubation, after which time no net incorporation occurred. Parasites grew normally from ring to schizont stages even in the absence of adenosine from the dilution medium.