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Serum Proteins Promoting [ 3 H]Thymidine Uptake by Trypanosoma (Schizotrypanum) cruzi (Chagas) in vitro
Author(s) -
O'DALY JOSE A.
Publication year - 1975
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1975.tb05231.x
Subject(s) - polyacrylamide gel electrophoresis , immunoelectrophoresis , in vitro , trypanosoma cruzi , thymidine , gel electrophoresis , microbiology and biotechnology , biology , blood proteins , immunodiffusion , biochemistry , chemistry , chromatography , antibody , immunology , parasite hosting , world wide web , computer science , enzyme
SYNOPSIS. Five proteins capable of stimulating [ 3 H]thymidine uptake by Trypanosoma cruzi in vitro were isolated from fetal calf serum by (NH 4 ) 2 SO 4 precipitation and ion exchange column chromatography. The proteins were partially characterized by immunodiffusion, immunoelectrophoresis, polyacrylamide gel disc electrophoresis, and SDS electrophoresis. As estimated by SDS electrophoresis, using 4 standards, the molecular weight of protein 1 was 100,000, that of protein 2 was 76,000. and that of proteins 3–5 was 68,000 daltons.