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Fine Structure of the Oocyst Walls of Isospora serini and Isospora canaria and Excystation of Isospora serini From the Canary, Serinus canarius L.
Author(s) -
SPEER CLARENCE A.,
DUSZYNSKI DONALD W.
Publication year - 1975
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1975.tb05212.x
Subject(s) - isospora , biology , anatomy , microbiology and biotechnology , feces
SYNOPSIS. Oocysts of Isospora serini and Isospora canaria , from the canary Serinus canarius , were broken, added to a cell suspension, fixed in Karnovsky's fluid, and studied in the electron microscope. The oocyst wall of each species had an electronlucent inner layer, a more osmiophilic middle layer and an outer layer of electron‐lucent ( I. serini ) or electron‐dense material interspersed with some electron‐lucent material ( I. canaria ). A few, relatively large lipid‐like bodies were present in the outer or middle layer of the oocyst wall of I. canaria. As many as 9 membranes were present in the oocyst wall of I. canaria and 3 in that of I. serini. When exposed to a trypsin‐sodium taurocholate fluid, sporozoites of I. serini excysted from 5‐month‐old sporocysts in vitro , but not from sporocysts stored for more than 6 months. No excystation occurred in 15‐month‐old I. canaria sporocysts. Similarities and differences in excystation between I. serini and other Isospora, Eimeria , and Sarcocystis species are discussed.