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Ultrastructure of the Invasion of Eimeria magna Sporozoites into Cultured Cells * † ‡
Author(s) -
JENSEN JAMES B.,
HAMMOND DATUS M.
Publication year - 1975
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1975.tb05193.x
Subject(s) - ruthenium red , vacuole , biology , eimeria , ultrastructure , electron microscope , staining , cell , microbiology and biotechnology , chemistry , anatomy , cytoplasm , biochemistry , physics , genetics , organic chemistry , optics , calcium
SYNOPSIS. Monolayers of bovine kidney cells were overlaid with Eimeria magna sporozoites and observed with phase‐contrast optics until penetration of the cells by the parasites had begun. Cells and penetrating parasites were fixed with glutaraldehyde and OsO 4 ‐containing ruthenium red, dehydrated, and embedded in situ . Cells being penetrated were selected for study in the electron microscope. The lack of intracellular staining with ruthenium red and intact plasmalemmas of cells being penetrated, was accepted as evidence that the sporozoites did not disrupt the plasma membranes. The sporozoite caused invagination of the host cell plasmalemma until the parasite was entirely within the cell, after which the invagination was sealed off by short pseudopodia enclosing the sporozoite within a membrane‐lined vacuole inside the cell. Often myelin‐forms, apparently of host cell origin, were seen in the space between the sporozoite and the cell.