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A Macromolecule‐Free Partially Defined Medium for Trypanosoma cruzi *
Author(s) -
YOSHIDA NOBUKO
Publication year - 1975
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1975.tb00957.x
Subject(s) - trypanosoma cruzi , macromolecule , hemin , biochemistry , chemistry , dialysis , biology , medicine , parasite hosting , computer science , heme , enzyme , world wide web
SYNOPSIS. A macromolecule‐free medium, containing in its defined part 3 salts, glucose, hemin, 21 amino acids, 3 lipids, and some undefined components obtained by dialysis of liver infusion, was developed for serial cultivation of Trypanosoma cruzi at 28 C. The medium allows prolonged cultivation of T. cruzi by serial transfers and growth comparable to that obtained in more complex media, including those containing blood serum.