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Isolation of Kinetoplast‐Mitochondrial Complexes from Leishmania tarentolae *
Author(s) -
BRALY PATRICIA,
SIMPSON LARRY,
KRETZER FRANK
Publication year - 1974
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1974.tb03752.x
Subject(s) - kinetoplast , leishmania , tris , mitochondrion , mitochondrial dna , chemistry , biochemistry , biophysics , microbiology and biotechnology , biology , dna , parasite hosting , world wide web , computer science , gene
SYNOPSIS. Kinetoplast‐mitochondrial complexes were liberated from Leishmania tarentolae by passing hypotonically swollen cells in dilute Tris‐EDTA through a needle at 100 1bs/in 2 . The complexes formed an equilibrium band by flotation in Renografin gradients at a density of 1.22 g/ml. The band was monitored by several mitochondrial and kinetoplastic markers: [ 3 H]DNA, succinate‐cytochrome c reductase activity, [ 50 Fe]hemoproteins and optical density at 600 nm. Electron microscopy showed that the sole component of the 1.22 g/ml band was the kinetoplast‐mitochondrial complex.