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Beta‐Oxidation in Glyoxysomes from Euglena *
Author(s) -
GRAVES LYNN B.,
BECKER WAYNE M.
Publication year - 1974
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1974.tb03750.x
Subject(s) - glyoxysome , euglena gracilis , euglena , biochemistry , glyoxylate cycle , thiolase , biology , enzyme , microbody , chemistry , dehydrogenase , chloroplast , catalase , gene
SYNOPSIS. We demonstrated previously the presence of glyoxysomes containing the glyoxylate cycle enzymes in Euglena gracilis grown in the dark on ethanol. We have now established that the glyoxysomes of Euglena grown on hexanoate also contain the following enzymes of the pathway for β ‐oxidation of fatty acids: hexanoyl‐CoA synthetase, 3‐ β ‐hydroxyacyl‐CoA dehydrogenase and thiolase. Estimations of specific activities indicate that these enzymes are over 20 times as active in glyoxysomes as they are in mitochondria, suggesting that the β ‐oxidation of fatty acids occurs almost entirely in Euglena glyoxysomes under these conditions. Thus, the entire portion of the gluconeogenic pathway from fatty acid to succinate is localized in the glyoxysome of Euglena.