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Morphometric Studies of Mitochondria in Tetrahymena pyriformis Exposed to Chloramphenicol or Ethidium Bromide *
Author(s) -
KAY EDWIN,
ROHATGI KALPANA,
KRAWIEC STEVEN
Publication year - 1974
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1974.tb03712.x
Subject(s) - tetrahymena pyriformis , ethidium bromide , mitochondrion , tetrahymena , chloramphenicol , biology , microbiology and biotechnology , biochemistry , dna , antibiotics
SYNOPSIS Cultures of Tetrahymena pyriformis strain ST were exposed to 300 μg chloramphenicol/ml or 15 μg ethidium bromide/ml for 48 hr. Qualitative assessments of electron micrographs reveal that the abundance of mitochondrial cristae decreases greatly. By equating the spatial characteristics of the organism with those of a prolate spheroid, the distribution and abundance of mitochondria were quantified. Such characterizations reveal that the size of individual mitochondria decreases by 35–60% and that the number of mitochondria/cell increases ∼8 fold. The observations are discussed in terms of coordinated mitochondrial and nuclear genetic activities.