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Nucleic Acid Precursor Incorporation by Eimeria nieschulzi (Protozoa: Apicomplexa) and Jejunal Villus Epithelium *
Author(s) -
MAYBERRY LILLIAN F.,
MARQUARDT WILLIAM C.
Publication year - 1974
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1974.tb03710.x
Subject(s) - guanosine , nucleic acid , biology , rna , microbiology and biotechnology , thymidine , adenosine , incubation , dna , biochemistry , uridine , ribosomal rna , gene
SYNOPSIS Autoradiography was used to investigate incorporation of tritiated adenine, adenosine, guanosine and thymidine by Eimeria nieschulzi and rat jejunal villus epithelial cells. At 2 1/2 days postinoculation, parasitized and control tissues were incubated for 20 min in oxygenated Tyrode's solution (37 C, pH 7.5) containing 30 μCi/ml of each nucleic acid precursor. Treatment of tissues with ribonuclease revealed that E. nieschulzi incorporated label from [ 3 H]adenine primarily into RNA while that from [ 3 H]adenosine and [ 3 H]guanosine was present mainly in DNA. Label from [ 3 H]thymidine was not utilized by parasites. Host villus epithelial cells incorporated label from [ 3 H]purines primarily into RNA. Labeled cytoplasmic RNA was significantly increased in parasitized cells after incubation in [ 3 H]adenine. Tritiated nuclear RNA and cytoplasmic RNA were significantly decreased in parasitized cells after incubation in [ 3 H]adenosine. Incorporation of label from [ 3 H]guanosine was similar for parasitized and control cells. A small quantity of label from each [ 3 H]precursor was incorporated into DNA of villus epithelial cell nuclei.