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Antigenic Makeup of Subcellular Fractions of Trypanosoma cruzi
Author(s) -
SEGURA ELSA LEONOR,
CURA ESTELA N.,
PAULONE IRENE,
VASQUEZ CESAR,
CERISOLA JOSE A.
Publication year - 1974
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1974.tb03703.x
Subject(s) - trypanosoma cruzi , microsome , biochemistry , acid phosphatase , ouchterlony double immunodiffusion , precipitin , antigen , biology , mitochondrion , cell fractionation , microbiology and biotechnology , trypanosoma , cell , immunodiffusion , enzyme , chemistry , virology , antiserum , immunology , parasite hosting , world wide web , computer science
SYNOPSIS Trypanosoma cruzi epimastigotes from cultures were separated into nuclear, mitochondrial, lysosomal, microsomal, and cell‐sap fractions. Enzymic and ultrastructutal controls served to determine the cleanness of separation. The bulk of the DNA was in the nuclear (78%) and mitochondrial (12%) fractions. RNA was found in microsomal (74%) and cell‐sap (14%) fractions. Marker enzyme distribution (succinic dehydrogenase, acid phosphatase, and glucose‐6‐phosphatase) was compared with their distribution in mammalian tissues. Subcellular localization of antigens by Ouchterlony tests revealed 2 specific precipitin lines in cell sap and 1 in the microsomal fraction. These antigens may prove diagnostically and immunoprotectively valuable.