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A Fluid System for the Cultivation, Light Microscope Examination and Manipulation of Obligate Anaerobes *
Author(s) -
RITTER HOPE
Publication year - 1974
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1974.tb03700.x
Subject(s) - obligate anaerobe , anaerobic exercise , obligate , microscope , atmosphere (unit) , environmental science , materials science , optics , biology , ecology , physics , meteorology , physiology
SYNOPSIS A chamber in tandem with oxygen‐free gas supply and gas‐collecting reservoirs constitutes a system capable of anaerobic maintenance within the range of 0.8–20 μM at 13 C. Cultures contained within the chamber portion of this system are examined through exposed oculars of a microscope otherwise sealed from room atmosphere. Materials are interchanged between the 2 atmospheres through an air lock. Shoulder‐length gloves enable all manipulations common to aerobic cultivation procedures without undue restraint.