Premium
Delayed in vitro Utilization of Glucose by Leishmania tropica Promastigotes *
Author(s) -
MUKKADA ANTONY J.,
SCHAEFER FRANK W.,
SIMON MICHAEL W.,
NEU CAROL
Publication year - 1974
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1974.tb03676.x
Subject(s) - pyruvate kinase , leishmania tropica , phosphofructokinase , hexokinase , biochemistry , in vitro , fructose , leishmania , adenosine monophosphate , chemistry , biology , kinase , glycolysis , enzyme , parasite hosting , world wide web , computer science
SYNOPSIS Leishmania tropica promastigotes do not utilize glucose provided in the medium until late log phase. Rapid depletion of glucose from the medium, however, occurs during late log and stationary phases. At about the same time, the cells show maximal rates of glucose uptake as well as peak levels of phosphofructokinase and pyruvate kinase activities. The glucose analog, 2‐deoxy‐D‐glucose inhibits glucose transport. Incorporation of this analog in the growth medium results in inhibition of growth. The hexokinase of L. tropica phosphorylates 2‐deoxy‐D‐glucose. Pyruvate kinase is activated by fructose‐1, 6‐diphosphate and adenosine monophosphate.