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Localization of Some Enzymes of β‐oxidation of Fatty Acids in the Peroxisomes of Tetrahymena *
Author(s) -
BLUM J. J.
Publication year - 1973
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1973.tb03600.x
Subject(s) - peroxisome , thiolase , biochemistry , tetrahymena pyriformis , tetrahymena , dehydrogenase , mitochondrion , isocitrate dehydrogenase , biology , isocitrate lyase , microbody , enzyme , organelle , glyoxylate cycle , gene
SYNOPSIS Mitochondria and peroxisomes were prepared from homogenates of Tetrahymena pyriformis by sedimentation through sucrose gradients. Catalase and isocitrate lyase served as peroxisomal markers; lactic dehydrogenase and glutamic dehydrogenase as mitochondrial markers. Acetyl‐CoA synthetase, octanoyl‐CoA synthetase, palmitoyl‐CoA synthetase, 3‐ β hydroxyacyl CoA dehydrogenase, and thiolase activities were found in both the peroxisomes and the mitochondria. It is suggested that β ‐oxidation of fatty acids accurs in both organelles in Tetrahymena.