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Immunogenicity and Infectivity of Sporozoites of Mammalian Malaria Isolated by Density‐Gradient Centrifugation *
Author(s) -
KRETTLI ANTONIANA,
CHEN DAVID H.,
NUSSENZWEIG RUTH S.
Publication year - 1973
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1973.tb03594.x
Subject(s) - plasmodium berghei , infectivity , immunogenicity , virology , biology , malaria , centrifugation , plasmodium (life cycle) , microbiology and biotechnology , antibody , sucrose gradient , parasite hosting , immunology , virus , biochemistry , membrane , world wide web , computer science
SYNOPSIS Sporozoites of rodent and simian malaria ( Plasmodium berghei and P. cynomolgi ) were purified by centrifugation on a linear Renografin/BSA gradient. This procedure made it possible to process rapidly a large number of infected mosquitoes leading to the recovery of a considerable proportion of sporozoites. Gradient‐recovered sporozoites (GRS) freed of most bacteria and mosquito tissue contaminants, retained their infectivity and immunogenicity. Mice repeatedly injected i.v. with irradiated GRS of P. berghei acquired total protection against an otherwise lethal sporozoite challenge. GRS of P. berghei and P. cynomolgi induced antisporozoite (CSP) antibody production in rats.