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Synthesis of RNA, Protein, Cellulose, and Mucopolysaccharide and Changes in the Chemical Composition of Hartmannella culbertsoni During Encystment under Axenic Conditions *
Author(s) -
RAIZADA M. K.,
MURTI C. R. KRISHNA
Publication year - 1972
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1972.tb03562.x
Subject(s) - axenic , cycloheximide , glucosamine , biochemistry , rna , cellulose , glycosaminoglycan , microbiology and biotechnology , taurine , leucine , chemistry , biology , protein biosynthesis , bacteria , amino acid , genetics , gene
SYNOPSIS Hartmannella culbertsoni trophozoites are transformed into viable cysts on exposure to a non‐nutrient agar medium containing 15 mM MgCl 2 and 20 mM taurine. Amebae differentiating in this encystment medium incorporate more uracil‐2‐ 14 C into RNA and more leucine‐1‐ 14 C or valine‐1‐ 14 C into proteins than controls. Encysting organisms incorporate significantly more glucose‐U‐ 14 C into cellulose and glucosamine‐1‐ 14 C into mucopolysaccharides. Incorporation of glucose‐U‐ 14 C into cellulose and of glucosamine‐1‐ 14 C into mucopolysaccharides are inhibited by actinomycin D or cycloheximide.