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Infectivity and Recovery of Tetrahymena pyriformis Strain S from Adult Female Cockroaches ( Periplaneta americana ) *
Author(s) -
SEAMAN GERALD R.,
TOSNEY T.,
BERGLUND RICHARD,
GOLDBERG GEORGE
Publication year - 1972
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1972.tb03549.x
Subject(s) - periplaneta , cockroach , biology , tetrahymena pyriformis , dictyoptera , tetrahymena , blattidae , protozoa , strain (injury) , axenic , infectivity , ciliate , lethargy , elongation , anatomy , inoculation , botany , ecology , horticulture , virology , microbiology and biotechnology , medicine , genetics , virus , materials science , bacteria , ultimate tensile strength , metallurgy
SYNOPSIS Hemocoelomic injection of 5,800–8,000 cells of Tetrahymena pyriformis strain S per female cockroach resulted in lethargy of the insects within 24 hr and death within 72 hr. Ciliates could be recovered 24–48 hr after injection of these amounts of ciliates. Inoculation of smaller numbers of cells resulted in no apparent ill effects to the insects and ciliates could not be recovered. After recovery from cockroaches and reestablishment in axenic culture, the ciliates were rounded and contained large numbers of lipid droplets which decreased on continued cultivation. The ciliates underwent a sequence of morphologic alterations involving a decrease in length and width to form ovoid cells, an approach to the pyriform shapes (but smaller than normal size), an elongation to an abnormal length/width ratio, and then, after 6 days in culture, a return to the normal shape with usual dimensions.