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Temperature‐Enhanced Osmotic Growth Requirement of Crithidia * †
Author(s) -
ELLENBOGEN BARBARA B.,
HUTNER S. H.,
TAMBURRO KATHLEEN M.
Publication year - 1972
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1972.tb03474.x
Subject(s) - crithidia fasciculata , mannitol , glycerol , osmotic pressure , sorbitol , osmotic shock , biochemistry , glycine , pentaerythritol , osmosis , chemistry , membrane , amino acid , dna , organic chemistry , fire retardant , gene
SYNOPSIS. An osmotic growth requirement for the trypanosomatid Crithidia fasciculata became conspicuous at 32 C. Osmo‐supportive compounds were surveyed at 28 vs. 33 C, in “low‐osmotic” defined medium. Effective osmotic support was provided by many compounds, e.g., glycerophosphate, sorbitol, mannitol, glycerol, Na isethionate, glycine, arginine, KCl, NaCl, NH 4 Cl, and K 2 SO 4 . The nonspecificity of the requirement was thus evident, but inactivity of the presumably poorly adsorbable pentaerythritol indicated that osmotic pressure was a likely but insufficient condition for satisfying the temperature‐enhanced growth requirement most clearly expressed as a need for osmotic support. Fortification of the medium with a combination of glycerophosphate, glycine, glycerol, and Tween 80 permitted good growth at 35 C. Possible relations between the temperature‐enhanced osmotic requirement cell membrane damage, and morphological phases of Trypanosoma and Leishmania are discussed.