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Carbohydrate‐Free Media for Crithidia
Author(s) -
TAMBURRO KATHLEEN M.,
HUTNER S. H.
Publication year - 1971
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1971.tb03394.x
Subject(s) - crithidia fasciculata , carbohydrate , biochemistry , sucrose , crithidia , sorbitol , biology , glycerol , chemistry , botany , protozoa , enzyme
SYNOPSIS. The insect trypanosomatid Crithidia fasciculata grew well at pH 3.8–6.3 in defined carbohydrate‐free media containing arginine (an essential amino acid) + proline + glutamic acid as substrates; glycerol was effective by itself. Precipitation of hemin in the acid media did not hinder growth. Further addition of succinic acid permitted growth matching that with carbohydrate. At pH 6.9–7.5 growth in this medium without carbohydrate with the aforementioned non‐fermentable substrates was slight; added carbohydrate (as sucrose or sorbitol) permitted good growth. Utilization of non‐carbohydrate substrates may contribute to Crithidia 's ability (and presumably to that of pathogenic trypanosomatids as well) to multiply in the insect gut and to the ability of some Trypanosoma species to multiply in the insect hemocoel and salivary gland.