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Aerobic Respiration of Kappa Particles from Paramecium aurelia , Stock 51 *
Author(s) -
KUNG CHING
Publication year - 1971
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1971.tb03326.x
Subject(s) - paramecium aurelia , kappa , respiration , sucrose , stock solution , chemistry , biochemistry , biology , zoology , paramecium , botany , linguistics , philosophy
SYNOPSIS. Kappa particles from killer cultures of stock 51 Paramecium aurelia were purified and their respiration measured polarographically. The slight bacterial contaminations in the kappa preparations were not significant. Freshly collected kappa in dilute buffer at room temperature had an endogenous Q O2 of 17.0 ± 1.6 μ l/mg dry weight/hr (mean ± standard error). The Q O2 decayed 50% in 5 hr. Among the sugars tested only glucose and sucrose increased the respiratory rate of kappa. The di‐ and tri‐carboxylates of the tricarboxylic acid cycle stimulated the respiration of kappa. KCN, CO and 2‐heptyl‐4‐hydroxyquinoline N ‐oxide (HOQNO) inhibited respiration. These findings ensure an organismic status for kappa and justify the belief that it is bacterial in origin.