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A New Method for Intraerythrocytic Cultivation of Malaria Parasites ( Plasmodium coatneyi and P. falciparum )
Author(s) -
TRAGER WILLIAM
Publication year - 1971
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1971.tb03314.x
Subject(s) - plasmodium falciparum , vial , parasite hosting , malaria , incubation , biology , virology , microbiology and biotechnology , immunology , chemistry , chromatography , biochemistry , world wide web , computer science
SYNOPSIS. Infected blood (from the rhesus monkey with P. coatneyi or an Aotus trivirgatus monkey with P. falciparum ) was placed in a special vial equipped with an overflow tube 2–3 mm above the bottom of the vial. A slow flow (50 ml per day) of culture medium was maintained over the settled layer of red cells. In favorable experiments both species of parasites went thru 2 cycles of development without addition of fresh erythrocytes. so that late schizonts and young rings were present after 4 as well as after 2 days of incubation. There was, however, no increase in total parasite number.