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Regulation of Cortical Proteins in Euplotes *
Author(s) -
RUFFOLO JOHN J.
Publication year - 1970
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1970.tb05169.x
Subject(s) - neuroscience , biology , computational biology , microbiology and biotechnology
SYNOPSIS. A method of isolating cortical organelles from single specimens of Euplotes eurystomus , involving lysis in an induced electric current, is described. The isolation technic was coupled with radioautography to study the patterns of incorporation and conservation of labeled proteins in the membranellar band (MB). Isolated single cells of known age were followed thru one or more divisions. A method of distinguishing between daughter cells (proters and opisthes) at division was utilized in some experiments. The old MB, which is apparently morphostatic, incorporates significant amounts of labeled proteins. The pattern of incorporation in total cell proteins at the 1st and 2nd divisions after pulse‐chase indicates that the levels of incorporation among daughter cells is equivalent. However, at the 1st division after pulse‐chase, the new (opisthe) MB is generally more heavily labeled than the old (proter) MB, and at the 2nd and 3rd divisions new MBs incorporate less label as their development is farther removed in time from the beginning of the chase. The higher level of incorporation in the MB of the 1st division opisthe is maintained thru subsequent divisions, indicating that in Euplotes proteins of the MB are relatively stable.