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Disappearance of Mating Reactivity in Paramecium caudatum upon Repeated Washing
Author(s) -
TAKAHASHI MIHOKO,
HIWATASHI KOICHI
Publication year - 1970
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1970.tb04746.x
Subject(s) - paramecium caudatum , reactivity (psychology) , paramecium , mating , chemistry , phase (matter) , potassium , biology , biophysics , chromatography , biochemistry , zoology , organic chemistry , medicine , alternative medicine , pathology
SYNOPSIS The appearance of mating reactivity of Paramecium caudatum was retarded by repeated washing of the cells in the logarithmic growth phase with Dryl's and other solutions. Highly reactive cells in the stationary phase also lost reactivity during the same treatment. Dryl's solution, sodium and potassium phosphate buffers, Miyake's physiologic balanced solution and exhausted culture medium were effective but deionized water saturated with CaCO 3 was ineffective. The addition of supernatant fluid from stationary phase cultures restored reactivity in 2 hr but was unable to do so when applied to extremely starved cells. These findings may be useful for study of the synthesis of mating‐type substances.