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Factors Affecting the Rate of Photophosphorylation by Isolated Chloroplasts of Euglena gracilis *
Author(s) -
CHANG IRENE C.,
KAHN J. S.
Publication year - 1970
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1970.tb04728.x
Subject(s) - photophosphorylation , euglena gracilis , chloroplast , euglena , chlorophyll , biology , photosynthesis , botany , chemistry , biochemistry , microbiology and biotechnology , gene
SYNOPSIS The rate of photophosphorylation by Euglena chloroplasts depends not only on the physiologic stage of cell growth but also on the stage of development of chloroplasts in these cells at the time of harvesting. Both of these processes can be markedly influenced by a number of environmental factors; they are affected neither in a parallel manner nor completely independent of each other. In addition, the rate of photophosphorylation of chloroplasts can also be greatly affected by the conditions employed for their isolation. After investigating the various environmental factors both during cell growth and chloroplast isolation, we have developed a procedure which increased the photophosphorylation rate of our chloroplast preparations more than 5‐fold, giving a specific activity in the range of 100‐150 μmoles ATP/mg chlorophyll/hr routinely. The procedure is simple, needs no special equipment and requires only 2 or 3 days for cell growth.