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Do Synchronizing Temperature Shifts Inhibit RNA Synthesis in Tetrahymena pyriformis? 1
Author(s) -
BYFIELD JOHN E.,
LEE YOUNG C.
Publication year - 1970
Publication title -
the journal of protozoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 0022-3921
DOI - 10.1111/j.1550-7408.1970.tb04712.x
Subject(s) - rna , tetrahymena pyriformis , tetrahymena , biology , in vivo , biochemistry , rna polymerase , in vitro , microbiology and biotechnology , biophysics , chemistry , genetics , gene
SYNOPSIS The effects of synchronizing temperatures on RNA metabolism in 2 strains of Tetrahymena pyriformis have been studied. High temperature shifts cause degradation of unstable RNA fractions, including whole cell RNA and nuclear RNA. The evidence indicates that this hydrolysis results in a net loss of unstable RNA during each temperature shift and, indirectly, to an inhibition of uptake 2 of extra‐cellular RNA precursors. The destruction of unstable RNA appears random; no evidence for stable template pools was found. In addition, both indirect in vivo and direct in vitro assays of RNA polymerase activity failed to reveal any inhibition by synchronizing heat shifts. The inhibition of precursor incorporation in vivo apparently stems from a lag in the uptake of label into the intracellular nucleotide pools. The temperature values required to induce net hydrolysis of unstable RNA correlate well with those required for synchronization for each strain.